Composite
Part:BBa_K1615069:Design
Designed by: Brooke Rothschild-Mancinelli Group: iGEM15_Edinburgh (2015-08-28)
CBDcenA+Linker fused to TVEL5 in RFC25
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1026
Illegal BglII site found at 1187
Illegal BamHI site found at 1250 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 832
Design Notes
Fusion done using RFC25.
Source
TVEL5 Laccase from Tramates versicolor. CBDcenA from Cellulomonas fimi.